Phytochemical Studies with Comparative Anti-Oxidant Analysis and Method Development Using Analytical Techniques for Adiantum Lunulatum

Authors- Phytochemical Studies with Comparative Anti-Oxidant Analysis and Method Development Using Analytical Techniques for Adiantum Lunulatum

Abstract-Due to the increasing demand for medicinal plants in both developed and developing countries, they depend on traditional plants because of their non-toxic, and lack of side effects. As compared to the other plant genera ferns which are one of the primitive plant groups are less studied. “Adiantum Lunulatum” also known as hamsapadi an Indian medicinal plant with a small leafy green. It is used to treat throat infections, febrile conditions etc. First Phytochemical Analysis was done which shows the presence of most of the secondary Metabolites like phenol, saponins, tannins, flavonoids, steroids, and anthocyanin. This plant is also reported to have high concentrations of antioxidants, possibly contributing to disease prevention following human consumption. We are checking the concentration of antioxidants in different parts of the plant ( Leaves & Midrib ). Following analysis of Adiantum lunulatum leaves and midrib, we report the antioxidant content potential of these species using two comparable techniques assessing the consistency between the assays – the ferric reducing antioxidant power (FRAP) assay and the Phosphomolybdate (PMA) assay. We conclude that there is variation in Adiantum lunulatum antioxidant potential and that ferric reducing antioxidant power (FRAP) assay and Phosphomolybdate (PMA) assay are useful techniques for measuring antioxidants in this plant. The extracts showed a considerable antioxidant effect in Ethanol & Acetone and Methanol & Acetone from two different assays respectively. Thin-layer chromatography reveals the presence of different metabolites of all plant extracts.

DOI: /10.61463/ijset.vol.13.issue1.125